single guide rna sgrna (Synthego Inc)

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Synthego Inc single guide rna sgrna
Single Guide Rna Sgrna, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/single+guide+rnas+sgrnas/sgrna/pmc13273111-269-6-22
Average 86 stars, based on 1 article reviews

single guide rna sgrna - by Bioz Stars, 2026-10

86/100 stars

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Synthesized:

Article Title: Disrupted lipid homeostasis as a pathogenic mechanism in ABCA7-associated Alzheimer's disease risk.
Article Snippet: .. Single guide RNAs (sgRNAs) were synthesized by Synthego, reconstituted in nuclease-free 1× TE buffer at 100 μM, pulse vortexed, and incubated on ice for 30 to 60 minutes before aliquoting and storing at−80◦C. .. To form ribonucleoprotein (RNP) complexes, 140 pmol of sgRNA was combined with 40 pmol of Cas9 nuclease (EnGen Spy Cas9NLS, Cat#M0646T, New England Biolabs) in Lonza P3 Nucleofector solution (Cat# V4XP-3032) at a 3.5:1 molar ratio, then incubated at room temperature (RT) for 10 minutes. iPSCs were cultured in mTeSR Plus medium (StemCell Technologies) on vitronectin-coated plates (Cat# A14700, Thermo Fisher Scientific) and pre-treated for at least 1 hour with 10 μM Y27632 and 2 μM thiazovivin to improve cell viability.

Article Title: The transcription factor CUX1 exerts opposing roles in human and mouse adipocyte differentiation.
Article Snippet: .. CRISPR-mediated gene knockout (KO) in APCs For each target gene, two specific single guide RNAs (sgRNAs) were designed in house and synthesized by Synthego. .. The sgRNAs and recombinant Cas9 protein were complexed to form ribonucleoproteins, which were then delivered into APC nuclei by electroporation using the 4D Nucleofector system (Lonza). sgRNA sequences are listed in Supplementary Table 2.

Article Title: Disrupted lipid homeostasis as a pathogenic mechanism in ABCA7 ‐associated Alzheimer's disease risk
Article Snippet: .. Single guide RNAs (sgRNAs) were synthesized by Synthego, reconstituted in nuclease‐free 1× TE buffer at 100 μM, pulse vortexed, and incubated on ice for 30 to 60 minutes before aliquoting and storing at −80°C. .. To form ribonucleoprotein (RNP) complexes, 140 pmol of sgRNA was combined with 40 pmol of Cas9 nuclease (EnGen Spy Cas9 NLS, Cat# M0646T, New England Biolabs) in Lonza P3 Nucleofector solution (Cat# V4XP‐3032) at a 3.5:1 molar ratio, then incubated at room temperature (RT) for 10 minutes. iPSCs were cultured in mTeSR Plus medium (StemCell Technologies) on vitronectin‐coated plates (Cat# A14700, Thermo Fisher Scientific) and pre‐treated for at least 1 hour with 10 μM Y27632 and 2 μM thiazovivin to improve cell viability.

Incubation:

Produced:

Article Title: O -GlcNAcylation of AMFR stabilizes TSPAN4 to regulate migrasome formation for viral release
Article Snippet: .. Single guide RNAs (sgRNAs) were produced using Synthego ( https://www.synthego.com/products/bioinformatics/crispr-design-tool ), and annealed and ligated into BsmBI-cut pLenti-CRISPR-V2 vectors. ..

Knock-Out:

Article Title: Secretome translation shaped by lysosomes and lunapark-marked ER junctions
Article Snippet: .. To generate cells with targeted knockout of human LNPK , single guide RNAs (sgRNAs) were designed against LNPK (Synthego). .. Particles were tracked using Imaris spot tracker (Oxford Instrument) and corresponding bleached SunTag signal was analysed over time.

Article Title: Site-specific glycosylation of Sec24D and myoferlin recruit ERGIC to ER exit sites for collagen trafficking.
Article Snippet: .. Single guide RNAs (sgRNAs) for SEC24D knock-in (5’-TTATGAAAATATCATTCCAT-3’, designed with SYNTHEGO https://design.synthego.com/#/) and MYOF knock-out (#1: 5’- AR TI CL E IN P RE SS ACTGTCGGCCCTCAATCACT-3’) were subcloned into pSpCas9(BB)-2A-GFP (pX458, Addgene #48138) by the Duke Functional Genomics Facility. pX458 harboring sgRNA Sec24D#3 was reported previously30. ..

CRISPR:

Gene Knockout:

Injection:

Article Title: The Na/K-ATPase Alpha-1 Subunit Fine-Tunes Platelet P2Y12 Function and Mediates Sex-Dimorphism-Associated Thrombosis.
Article Snippet: .. Single guide RNAs (sgRNAs) targeting early exons of atp1a1 paralogs (Synthego) were mixed with Cas9 and injected into single-cell stage embryos. ..

Single Cell:

Knock-In:

Functional Assay:

other:

Article Title: The transcription factor CUX1 exerts opposing roles in human and mouse adipocyte differentiation
Article Snippet: Compensation was performed using CytExpert v2.4, and data were gated and visualized in FlowJo v10.

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